Accumulative researches have demonstrated the critical functions of long non-coding RNAs (lncRNAs) in the progression of malignant tumors, including bladder cancer (BC). Our previous studies showed that lnc-DILC was an important tumor suppressor gene in both liver cancer and colorectal cancer. However, the role of lnc-DILC in BC remains to be elucidated. In the present study, we for first found that lnc-DILC was downregulated in human bladder cancer tissues. Lnc-DILC overexpression suppressed the proliferation, metastasis and expansion of bladder cancer stem cells (CSCs). Mechanically, lnc-DILC suppressed BC cells progression via STAT3 pathway. Special STAT3 inhibitor S3I-201 diminished the discrepancy of growth, metastasis and self-renewal ability between lnc-DILC-overexpression BC cells and their control cells, which further confirmed that STAT3 was acquired for lnc-DILC-disrupted BC cell growth, metastasis and self-renewal. Taken together, our results suggest that lnc-DILC is a novel bladder tumor suppressor and indicate that lnc-DILC inhibits BC progression via inactivating STAT3 signaling.
Gene. 2019 Jun 06 [Epub ahead of print]
Qian-Yun Ma, Shu-Ying Li, Xi-Zhou Li, Teng-Fei Zhou, Yong-Fu Zhao, Fang-Lin Liu, Xiao-Na Yu, Jian Lin, Fan-Yue Chen, Jie Cao, Hui-Jun Xi, Heng-Yu Li
Department of Urology surgery, First Affiliated Hospital of Second Military Medical University, Shanghai 200433, China., Department of Rehabilitation Medicine, First Affiliated Hospital of Second Military Medical University, Shanghai, 200433, China., Department of Breast and Thyroid surgery, First Affiliated Hospital of Second Military Medical University, Shanghai 200433, China., Department of Internal Medicine, The No. 313 Hospital of PLA, Huludao 125000, China., Department of Internal Medicine, First Affiliated Hospital of JinZhou Medical University, JinZhou 121001, China., Department of Urology surgery, First Affiliated Hospital of Second Military Medical University, Shanghai 200433, China. Electronic address: ., Department of Gastroenterology, First Affiliated Hospital of Second Military Medical University, Shanghai 200433, China. Electronic address: ., Department of Breast and Thyroid surgery, First Affiliated Hospital of Second Military Medical University, Shanghai 200433, China. Electronic address: .