To investigate the correlations of the expression of micro ribonucleic acid (miR)-203 with the onset and prognosis of bladder cancer.
Human bladder cancer T24 cell line with miR-203 overexpression were constructed. Effects of miR-203 expression on cell proliferation and apoptosis were detected via methyl thiazolyl tetrazolium (MTT) assay and flow cytometry, respectively. Fifty-eight patients with bladder cancer treated in our hospital were selected, and para-carcinoma tissues were used as controls. The expression of miR-203 in bladder cancer tissues was detected via quantitative polymerase chain reaction (qPCR), and the survival time of patients with bladder cancer was recorded in detail. Moreover, correlations of miR-203 expression with onset and prognosis of patients were evaluated via multivariate Cox regression analysis.
Results of qPCR revealed that after T24 cells were transfected with miR-203, the miR-203 expression level in the overexpression group was significantly higher than that in the control group (p<0.01), the cell proliferation capacity was significantly lower than that in the control group (p<0.01), and the apoptotic level was also significantly higher than that in the control group (p<0.01). The miR-203 expression level in carcinoma tissues of patients with bladder cancer was obviously lower than that in the para-carcinoma tissues (p<0.01). The miR-203 expression had close correlations with the tumor size, differentiation grade and tumor stage (p<0.01), and the 5-year survival rate of patients in low miR-203 expression group was remarkably lower than that in the high miR-203 expression group (p<0.01). Besides, the results of multivariate Cox regression analysis revealed that the tumor grade of differentiation, tumor-node-metastasis (TNM) stage and miR-203 expression were closely related to the prognosis of patients (p<0.01).
The high expression of miR-203 can significantly reduce the proliferation and promote the apoptosis of bladder cancer cells. The increased expression level of miR-203 can effectively increase the survival rate and improve the prognosis of patients.
Journal of B.U.ON. : official journal of the Balkan Union of Oncology. 0000 Jan [Epub]
Zhongming Huang, Guanghua Liu
Department of Urology, Peking Union Medical College Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing 100730, China.