Question: Can a genomic biomarker estimate the risk of prostate cancer clinical endpoints in men who received salvage radiation for rising prostate-specific antigen levels after surgery?
Findings: In this ancillary study of 352 men randomized to placebo or hormone therapy in the NRG/RTOG 9601 clinical trial of salvage radiation, the Decipher genomic classifier was independently associated with the risk of metastasis, prostate cancer–specific mortality, and overall survival.
Meaning: These findings suggest that the Decipher genomic classifier is a promising biomarker to risk stratify men to better enable hormone therapy treatment decisions for biochemical recurrence of their prostate cancer after surgery.
Importance: Decipher (Decipher Biosciences Inc) is a genomic classifier (GC) developed to estimate the risk of distant metastasis (DM) after radical prostatectomy (RP) in patients with prostate cancer.
Objective: To validate the GC in the context of a randomized phase 3 trial.
Design, Setting, and Participants: This ancillary study used RP specimens from the phase 3 placebo-controlled NRG/RTOG 9601 randomized clinical trial conducted from March 1998 to March 2003. The specimens were centrally reviewed, and RNA was extracted from the highest-grade tumor available in 2019 with a median follow-up of 13 years. Clinical-grade whole transcriptomes from samples passing quality control were assigned GC scores (scale, 0-1). A National Clinical Trials Network–approved prespecified statistical plan included the primary objective of validating the independent prognostic ability of GC for DM, with secondary endpoints of prostate cancer-specific mortality (PCSM) and overall survival (OS). Data were analyzed from September 2019 to December 2019.
Intervention: Salvage radiotherapy (sRT) with or without 2 years of bicalutamide.
Main Outcomes and Measures: The preplanned primary endpoint of this study was the independent association of the GC with the development of DM.
Results: In this ancillary study of specimens from a phase 3 randomized clinical trial, GC scores were generated from 486 of 760 randomized patients with a median follow-up of 13 years; samples from a total of 352 men (median [interquartile range] age, 64.5 (60-70) years; 314 White [89.2%] participants) passed microarray quality control and comprised the final cohort for analysis. On multivariable analysis, the GC (continuous variable, per 0.1 unit) was independently associated with DM (hazard ratio [HR], 1.17; 95% CI, 1.05-1.32; P = .006), PCSM (HR, 1.39; 95% CI, 1.20-1.63; P < .001), and OS (HR, 1.17; 95% CI, 1.06-1.29; P = .002) after adjusting for age, race/ethnicity, Gleason score, T stage, margin status, entry prostate-specific antigen, and treatment arm. Although the originally planned analysis was not powered to detect a treatment effect interaction by GC score, the estimated absolute effect of bicalutamide on 12-year OS was less when comparing patients with lower vs higher GC scores (2.4% vs 8.9%), which was further demonstrated in men receiving early sRT at a prostate-specific antigen level lower than 0.7 ng/mL (−7.8% vs 4.6%).
Conclusions and Relevance: This ancillary validation study of the Decipher GC in a randomized trial cohort demonstrated association of the GC with DM, PCSM, and OS independent of standard clinicopathologic variables. These results suggest that not all men with biochemically recurrent prostate cancer after surgery benefit equally from the addition of hormone therapy to sRT.
Trial Registration ClinicalTrials.gov identifier: NCT00002874
Authors: Felix Y. Feng, MD; Huei-Chung Huang, MA; Daniel E. Spratt, MD; Shuang (George) Zhao, MD; Howard M. Sandler, MD; Jeffry P. Simko, MD, PhD; Elai Davicioni, PhD; Paul L. Nguyen, MD; Alan Pollack, MD, PhD; Jason A. Efstathiou, MD, PhD; Adam P. Dicker, MD, PhD; Tamara Todorovic, MSc; Jennifer Margrave, BSc; Yang (Seagle) Liu, PhD; Bashar Dabbas, MD; Darby J. S. Thompson, PhD; Rajdeep Das, MD, PhD; James J. Dignam, PhD; Christopher Sweeney, MD; Gerhardt Attard, PhD; Jean-Paul Bahary, MD; Himanshu R. Lukka, MD; William A. Hall, MD; Thomas M. Pisansky, MD; Amit B. Shah, MD; Stephanie L. Pugh, PhD; William U. Shipley, MD; Phuoc T. Tran, MD, PhD
Department of Radiation Oncology, UCSF Medical Center, San Francisco, California (Feng, Das); Department of Medicine, UCSF Medical Center, San Francisco, California (Feng, Das); Department of Urology, UCSF Medical Center, San Francisco, California (Feng, Das); Decipher Biosciences, San Diego, California (Huang, Davicioni, Todorovic, Margrave, Liu, Dabbas); Department of Radiation Oncology, University of Michigan, Ann Arbor (Spratt, Zhao); Department of Radiation Oncology, Cedars-Sinai Medical Center, Los Angeles, California (Sandler); NRG Biorepository, Department of Pathology, UCSF Medical Center, San Francisco, California (Simko); Department of Radiation Oncology, Brigham and Women’s Hospital, Boston, Massachusetts (Nguyen); Department of Radiation Oncology, University of Miami Miller School of Medicine, Miami, Florida (Pollack); Department of Radiation Oncology, Massachusetts General Hospital, Boston, Massachusetts (Efstathiou, Shipley); Department of Radiation Oncology, Thomas Jefferson University Hospital, Philadelphia, Pennsylvania (Dicker); Emmes Canada, Vancouver, British Columbia, Canada (Thompson); NRG Oncology Statistics and Data Management Center, Philadelphia, Pennsylvania (Dignam, Pugh); Department of Public Health, University of Chicago, Chicago, Illinois (Dignam); Department of Medicine, Dana-Farber/Harvard Cancer Center, Boston, Massachusetts (Sweeney); Department of Oncology, University College London, London, United Kingdom (Attard); Department of Radiation Oncology, Centre Hospitalier de l’Université de Montréal-Notre Dame, Montreal, Quebec, Canada (Bahary); Department of Radiation Oncology, Juravinski Cancer Centre at Hamilton Health Sciences, Hamilton, Ontario, Canada (Lukka); Department of Radiation Oncology, Froedtert and the Medical College of Wisconsin, Madison, Wisconsin (Hall); Department of Radiation Oncology, Mayo Clinic, Rochester, Minnesota (Pisansky); Department of Radiation Oncology, WellSpan Health-York Cancer Center accruals under Thomas Jefferson University Hospital, Philadelphia, Pennsylvania (Shah); Department of Radiation Oncology, Johns Hopkins University, Baltimore, Maryland (Tran); Department of Oncology, Johns Hopkins University, Baltimore, Maryland (Tran); Department of Urology, Johns Hopkins University, Baltimore, Maryland (Tran).
Source: Feng F, Huang HC, Spratt D et al.”Validation of a 22-Gene Genomic Classifier in Patients With Recurrent Prostate Cancer An Ancillary Study of the NRG/RTOG 9601 Randomized Clinical Trial.” JAMA Oncology. 2021. 2020.7671.