Radiation therapy is a cornerstone of bladder-sparing treatment regimens for patients with muscle-invasive bladder cancer (MIBC). However, a significant number of MIBCs are radioresistant. Components of tumor immune microenvironment (TIME) can mediate tumor radioresistance. Polymorphonuclear neutrophils (PMNs) are known to infiltrate the TIME post-radiation and are associated with adverse outcomes for patients with MIBC. PMNs extrude web-like structures composed of DNA and histones, known as neutrophil extracellular traps (NETs), but it is unclear if these NETs play a role in response to radiation.