The most common type of male infertility is asthenospermia. We cloned DnaJ heat shock protein family member B13 (Dnajb13/DNAJB13), a type II HSP40 family member that is highly expressed in the testis. DNAJB13 plays a crucial role in sperm flagellar function.
The aim of this study was to investigate whether a correlation exists between DNAJB13 and low sperm motility in infertile men.
In the present study, we performed a mutation screening of the DNAJB13 gene in 92 idiopathic asthenozoospermia patients and 200 men with normal fertility. Additionally, we used immunoelectron microscopy, co-immunoprecipitation, mass spectrometric detection, indirect immunofluorescence assay, transmission electron microscopy studies, isobaric tags for relative and absolute quantitation, and multiple reaction monitoring studies to analyze changes in DNAJB13 protein.
A novel c.106T>C mutation of DNAJB13 was present in nearly 10% (9/92) of idiopathic asthenozoospermia patients and was absent in 200 fertile men. A computer-assisted sperm analyzer and transmission electron microscopy analysis using samples from 9 patients with DNAJB13 mutations demonstrated that most spermatozoa were immotile due to sperm tail defects. Multiple reaction monitoring results indicated that DNAJB13 protein levels were reduced after gene mutation. We achieved a pregnancy rate of 100% in 8 patients with DNAJB13 mutations using ICSI.
The DNAJB13 heterozygous variant may affect fertility. ICSI can help these patients with low fertility to father children.
Andrology. 2019 Jul 25 [Epub ahead of print]
W N Li, L Zhu, M M Jia, S L Yin, G X Lu, G Liu
Hunan Guangxiu Hi-tech Life Technology Co., Ltd., Changsha, China., The Xiangtan Central Hospital, Yuhu District, Xiangtan, China., Shanxi Provincial Maternal and Child Health Care Hospital, Yanta District, Xi’an, China., Reproductive and Genetic Hospital of CITIC-Xiangya, Changsha, China., The Institute of Reproduction and Stem Cell Engineering, Central South University, Changsha, China.