Liquid biopsy is becoming increasingly important as a guide for selecting new drugs and determining their efficacy. In urological cancer, serum markers for renal cell and urothelial cancers has made the development of liquid biopsy for these cancers strongly desirable. Liquid biopsy is less invasive than conventional tissue biopsy is, enabling frequent biopsies and, therefore, is considered effective for monitoring the treatment course. Circulating tumor cells (CTCs) are a representative liquid biopsy specimen. In the present study, we focused on developing our novel technology for capturing renal cell cancer (RCC)-CTCs using an anti-G250 antibody combined with new devices. Basic experiments of our technology showed that it was possible to detect RCC-CTC with a fairly high accuracy of about 95%. Also, RCC-CTC was identified in the peripheral blood of actual RCC patients. Additionally, during the treatment course of the RCC patient, change in the number of RCC-CTC was confirmed in one case. We believe that the technology we developed will be useful for determining the treatment efficacy and drug selection for the treatment of renal cell cancer (RCC). In order to solve issues such as thresholds setting of this technology, large-scale clinical trials are expected.

Diagnostics (Basel, Switzerland). 2019 Aug 14*** epublish ***

Michio Naoe, Chiho Kusaka, Mika Ohta, Yuki Hasebe, Tsutomu Unoki, Hideaki Shimoyama, Takehiko Nakasato, Kazuhiko Oshinomi, Jun Morita, Kohzo Fuji, Yoshio Ogawa, Mana Tsukada, Masataka Sunagawa, Hikaru Ishii

Department of Urology, Showa University, Tokyo 142-8666, Japan. ., Department of Urology, Showa University, Tokyo 142-8666, Japan., Department of Physiology, School of Medicine, Showa University, Tokyo 142-8666, Japan., Ishii-clinic Kyobashi Edogrand, Tokyo 104-0031, Japan.