To present and validate a new technique for biobanking fresh-frozen prostate cancer tissue based on MRI-transrectal ultrasound fusion biopsy.
From August 2014 to August 2016, patients with elevated levels of PSA and at least one suspicious lesion on MRI were invited to this study. Each MRI-suspicious lesion was biopsied repeatedly for at least two cores in the same location. These repeated cores were labelled A/A’, B/B’, etc. The A/B cores were submitted for histological assessment, and the corresponding A’/B’ cores were stored in an -80°C freezer for biobanking. Sixty biobanked samples were processed for histological assessment to compare their pathological parameters with their corresponding paraffin samples. Another 20 biobanked samples were processed for RNA quality evaluation.
Fifty-six of the 60 selected banking samples matched their corresponding paraffin samples for benign vs. malignant diagnosis, leading an overall concordance rate of 93.3%. There was no significant difference between banking samples and the corresponding paraffin samples in cancer percentage and Gleason score. The RNA Integrity Number (RIN) value ranged from 6.8 to 9.3 (mean 7.89).
The current study demonstrates that the histological identity of the banked prostate biopsy sample can be accurately predicted by its corresponding paraffin samples. MRI-TRUS fusion biopsy based biobanking method is highly efficient, timesaving and has high quality tissues both at the histological and RNA integrity levels.
Urology. 2019 Sep 02 [Epub ahead of print]
Changwei Ji, Wei Wang, Qun Lu, Yao Fu, Hao Jiang, Wei Chen, Hongqian Guo
Department of Urology, Drum Tower Hospital, Medical School of Nanjing University, Nanjing, Jiangsu, China; Institute of Urology, Nanjing University, Nanjing, Jiangsu, China., Department of Pathology, Drum Tower Hospital, Medical School of Nanjing University, Nanjing, Jiangsu, China., Department of Urology, Drum Tower Hospital, Medical School of Nanjing University, Nanjing, Jiangsu, China; Institute of Urology, Nanjing University, Nanjing, Jiangsu, China. Electronic address: .